Monochromatic (e.g., laser)

In a method of analytical spectrometry, material to be analysed is introduced in solution between a pair of charged electrodes so as to produce a light-absorbing atomic or molecular species by electrolysis at an electrode surface. A beam of light of an appropriate wavelength is directed through the solution...     

A spectrofluorimeter for measuring excitation and emission spectra uses a flash or pulsed light source, an excitation monochrometer adjustable by a stepping motor, a first optical system for directing radiation onto a sample under test, a reference light detector for monitoring the excitation radiation,...     

Method and apparatus for providing flash photolysis of CO-inhibited cytochrome oxidase in a first portion of a sample exposed to an oxygenating agent and optically comparing the result with a non-photolyzed second portion of the sample or with a previously photolyzed portion of the sample. The sample...     

A fluorescence spectrophotometer in which a monochromatic beam of radiation is directed from an excitation monochromator through a first optical system to a sample being evaluated. Fluorescence from the sample is collected by a second optical system and is directed to an emission monochromator. These...     

A system for suppressing normal serum fluorescence in fluorescence immunoassay, in which in one embodiment, the immunoassay fluorescently tagged reaction product in a single sample is irradiated at a first wavelength which will excite fluorescence from the tagged product and then at a different wavelength...     

An immune assay for tracing processes and reactions employs a narrow band laser tuned to the absorption line of a tracer isotope coupled chemically to a carrier molecule of protein. The carrier molecule is reacted with another molecule, the reaction products are vaporized, and resonance fluorescence...     

An onboard power supply and metering unit is connected, via an overboard cable e.g., 1000 meters long, to an underwater light-pulse projecting unit and a fluoroescence-sensing receiver unit. The operating power is transmitted to the underwater units by an onboard constant-current source, instead of a...     

An optical Raman microprobe with laser excitation for illuminating a sample, comprising means for illuminating the sample with a pulsed or continuous laser radiation of selected frequency, a microscope furnishing an enlarged image of the sample, means for selecting the wavelengths of the re-emitted or...     

The disclosure is directed to a cell analysis apparatus incorporating a paraboloidal cavity for maximum utilization for improved cell characteristic monitoring.

A sample of material to be analyzed is applied in a thin layer to a substrate such as an elongated tape and then is illuminated by a laser beam of such wavelength and power that at least a portion of the sample is vaporized. The substrate is non-absorptive at the laser wavelength and therefore is unaffected...     

Assay for a ligand wherein the ligand is contacted in a fluid with (1) labeled binder or (2) binder and labeled reference ligand, and the presence of ligand determined from the movement of labeled material in at least one region of the fluid. In a particular assay, the label is a fluorescent material...     

In fluorescence analysis of multiply stained particles, particularly biological cells, in a continuous flowthrough procedure, in which the particles are suspended in a carrier fluid, the carrier fluid containing the suspended particles is encased in a sheath stream, the resulting composite stream is...     

An apparatus for an optical measurement of the concentration of substances ncluding at least one monochromator means, one photometer means and one optometric means. A further reference indicator means is added to the indicator means. This further indicator means changes the measuring light and will not...     

Saturation spectroscopy involving synchronous phase-sensitive detection and measurement of the intensity of the output when one of the input beams is chopped has a drawback that scattered light and fluorescence from the sample will also be chopped and produce a large background signal. According to the...     

Flow systems for photometric analysis of particles, such as biological cells, particularly for obtaining multidmensional slit-scan type contours, as well as other images. In one broad approach, fluorochrome-stained cells in suspension flow through a focused slit of laser excitation light defining a planar...     

In order to provide a spectrofluorometer which measures the emission polarization spectrum of a sample at high precision and with ease, a first spectrum signal I.sub.HV (.lambda..sub.EM) and a second spectrum signal I.sub.HH (.lambda..sub.EM) are formed by scanning the wavelength of an emission monochromator....     

A first monochromator includes a diffraction grating that is adapted for occlusion by a mirror. When the mirror occludes the grating, a white light input is transmitted through the first monochromator and through a sample under test, thereby causing a transmission of non-absorbed light from the sample....     

A fluorescence spectrophotometer comprising a light source, an excitation side monochromator which makes light from the light source be subjected to spectroscopic analysis for illuminating as actinic light a sample, a fluoroscence side monochromator which makes fluorescence light from the sample be subjected...     

A method for accurately monitoring and adjusting gas phase processes such as gas etching and chemical vapor deposition has been found. This method relies on the use of induced fluorescence. The gaseous phase used in the process to be monitored is probed by excitation with a suitable energy source. The...     

A multiple-reflection optical cell for Raman or fluorescence gas analysis consists of two spherical mirrors positioned transverse to a multiple-pass laser cell in a confronting plane-parallel alignment. The two mirrors are of equal diameter but possess different radii of curvature. The spacing between...     

Fluoroimmunoassay of fluorescently tagged species using a fiber optical light guiding system, and single or paired fluorescent detectors, and polarizing the activating radiation and both fluorescent radiation outputs and apparatus for carrying out such assay is disclosed.

The invention relates to a spectral fluorometer arrangement including a laser light source, particularly for use in measuring the luminescence in the course of a qualitative and quantitative determination of a sample material, comprising a beam splitter inserted between said laser light source and a...     

The present invention relates to an apparatus for laser isotope detection and concentration measurement (LIDAM). A test sample containing first and second isotopes of a specific material or compounds formed with the first and second isotopes of the specific material is placed in an optical bridge with...     

A radiation fringe velocimeter subsystem and a subsystem for determining air density related parameters, are combined into a single optical system (20) which permits various important parameters associated with the operation of an aircraft to be measured at a sample location at a sufficient distance...     

The invention relates to a spectral fluorometer arrangement including at least one laser light source, particularly for use in measuring the luminescence in the course of a qualitative and quantitative determination of a sample material, the region of which to be excited is arranged in the object point...     

A sensitive frontal approach fluorometer which is suitable for measuring the fluorescence of samples in open top microtest wells and which has an optical system for (a) directing an exciting light downwardly into the well's open top to fluorescently excite the sample and (b) detecting the sample's emitted...     

A fluorimeter comprises an excitation optical system projecting the excitation light having a selected wavelength for a sample to said sample, a sample cell for accommodating the sample; fluorescent light sensors for sensing the fluorescent light emitted from the sample; a transmission light sensor for...     

Arrangement for measuring the fluorescence polarization of cells or particles which are aligned individually along a flow stream illuminated by means of an illumination field wherein the cells are passed in succession through two light beams so as to excite fluorescence; the directions of polarization...     

Fluorescent measurements are taken utilizing a Raman enhancing surface by a method which allows reduction or essentially complete elimination of interference by fluorescence quenchers. In particular, the fluorescent medium to be measured is interacted with a Raman enhancing surface. Through this interaction...     

A double monochrometer comprises a main monochrometer including a plane grating and an auxiliary monochrometer including a concave grating. The concave grating is disposed on the entrance or exit optical axis of the main monochrometer. Preferably, the angular dispersion of the concave grating is selected...     

An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump for delivering to the reaction cuvette a given amount of a given...     

A plasma monitor which measures the distribution of particular chemical species (atom, molecule, ion) in plasma generated inside an instrument making use of the plasma. Laser light having a particular wavelength is radiated to the particular chemical species so as to let it generate fluorescence. The...     

Induced fluorescence of air molecules is used to determine air data parameters such as barometric altitude. The air molecules at a sample location are caused to fluoresce by means of a suitable energy source. A detector having an effective field of view including the sample location measures the fluorescence...     

A laser Raman microprobe separates a laser beam reflected by a sample and a Raman scattered light generated by the sample by a filter which transmits or reflects a light in a predetermined wavelength region including a wavelength region of the reflected laser beam and applies only the Raman scattered...     

An arrangement for measuring diffusing particles has at least one monochromatic radiation source and a light measuring device, as well as at least one material limited particle-permeable indicator chamber provided with an optical fluorescent indicator which is optically changeable by the particles to...     

A method is described for measuring the uranium content of aqueous solutions wherein a uranyl phosphate complex is irradiated with a 5 nanosecond pulse of 425 nanometer laser light and resultant 520 nanometer emissions are observed for a period of 50 to 400 microseconds after the pulse. Plotting the...     

A temporal spectral photometer for use in obtaining spectral and temporal information simultaneously of an ultrafast pulse of luminescent light emitted from a sample upon excitation thereof includes an excitation section and a detection section. The excitation section includes a picosecond laser for...     

It has been shown that the use of a shutter to cut off the light beam entering the optical system of a fluoresence spectrophotometer will reduce UV deterioration of the components in the optical system. This invention discloses how control of the output of the light source by lowering its arc current...     

A software controlled automatic shutter is described which minimizes ultraviolet irradiation damage to optical components from a high intensity spectrophotometric light source. The shutter automatically opens when measurements are being made and closes when the photometer system is idle. The digital...     

A spectrophosphorimeter with a single spectrometer so as to obviate the need of a couple of spectrometers of conventional spectrophosphorimeters, wherein one spectrometer is operated as an excitation-side spectrometer only when the light is allowed to pass a light interceptor, and the other spectrometer...     

Apparatus and method for providing an optical detection of a binding reaction between a ligand and an antiligand, including, a pattern formed by a spatial array of microscopic dimensions of antiligand material, ligand material interacting with the antiligand material to produce a binding reaction between...     

A light detector which can be gated on and off over an ultrashort time window, such as in picoseconds or femtoseconds, is disclosed. The light detector includes, in one embodiment, an input slit for receiving a light signal, relay optics, a sweep generator and a tubular housing, the tubular housing having...     

An electrokinetic process and apparatus employing coherent radiation-excited fluorescence for detection is disclosed. In a preferred embodiment, the support liquid is a chiral liquid and the process is employed to separate and detect the separation of optical isomers.

A fluorescent spectral analysis apparatus comprises a source of a laser beam, and first and second optical fibers to be inserted in an endoscope with forward ends extended from a distal end of the endoscope. The first optical fiber guides the laser beam emitted from the source to a subject portion to...     

A self-contained automatic computer controlled system for checking and recalibrating the wavelength scale of both monochromators of a fluorescence spectrophotometer is described. No operator adjustments are required. The system sets the wavelength of each monochromator to within 0.1 nanometers. Besides...     

In order to improve the sensitivity and the spectral resolution in measuring the luminescence of diverse sample materials, adapted optical imaging relationships at variable excitation output are provided for the different optical paths of the luminescence light emitted by the sample material. An excitation...     

A method for separating diamonds from gangue in which discrete units of gangue are passed through a beam of laser radiation capable of causing Raman spectral activation. The scattered radiation is detected and caused to actuate an ejector which separates units of diamond containing gangue from units...     

This invention relates to a device for measuring fluorescence polarization, which permits to measure the degree of fluorescence polarization in a short time and also its variations in time. In order to achieve this object, a device for measuring fluorescence polarization according to this invention is...     

A laser communication system in which a filter-detector is provided which has a high acceptance angle and a narrow bandwidth. The filter-detector contains vaporized selected atomic species or a molecular composition dissociable to the selected atomic species. The selected atomic species electronic ground...     

There is disclosed herein a reference system for a fluorometer designed to detect very low levels of materials tagged with fluorophores. There is also disclosed an optical system for use in such a system which improves the signal to noise ratio. The reference system utilizes pulsed arc light excitation...     

An optical measuring system for measuring in situ by means of one or more fiber-optic probes reflection absorption fluorescence, phosphorescence and turbidity of a fluid or (for all but the last measurement) of a fluid-solid interface. High intensity pulsed light is conveyed to the measurement site by...     

An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump for delivering to the reaction cuvette a given amount of a given...     

A detection system is provided for identifying individual particles or molecules having characteristic emission in a flow train of the particles in a flow cell. A position sensitive sensor is located adjacent the flow cell in a position effective to detect the emissions from the particles within the...     

A dual wavelength spectrophotometer produces a relatively small, high power, high duty cycle light spot from a single relatively low power multi-chromatic light source. A Xenon arc lamp light source is focused by an ellipsoidal mirror onto a rotating partially reflective optical chopper. The chopper...     

This invention is for obtaining ratios of Raman spectrum by using a referential sample representing at least two of Raman spectral lines, and for correcting fluorescence intensities of the sample to measured on the basis of the obtained ratios of Raman spectrum.

An optical inspection system for using laser-induced luminescence to detect deterioration of a polymer-based material. The inspection system comprises an excitation means for illuminating a specimen of the polymer material to cause it to produce fluorescent radiation. The spectral representation of the...     

An apparatus is provided for remotely monitoring pH. A support material is provided on which organic dye molecules are covalently attached at a surface density falling within a predetermined range. The pH dependent fluorescence response of the bound organic dye molecules depends critically on surface...     

A system for detecting the radiant energy emitted from different closely spaced species includes two detectors each having a large entrance angle for receiving the radiant energy, and wavelength selective filters between the detector and species, the transmission vs. wavelength characteristics being...     

A high sensitivity fluorescence and absorption detector comprising an elongated substantially cylindrical flow cell connected to and receiving the outflow of a fluid chromatography column; a mechanism for projecting a fluorescence generating beam of electromagnetic energy along on an axis substantially...     

An optical sensor device uses surface plasmon resonance to detect the presence of a specific material. A transparent body (12) is coated with a thin gold film (14) which film may be coated e.g. with an antibody material. The arrangement is illuminated with a divergent light beam and light internally...     

An optical inspection system for using laser-induced luminescence to detect the quality of organic materials, such as peanuts. The inspection system comprises an excitation means for illuminating a specimen to cause it to produce fluorescent radiation. The spectral representation of the fluorescence...     

An electrical signal measuring device for measuring with high time resolution a relatively long optical phenomenon representing the electrical signal. A linear array of laser diodes is provided with an electrical signal and a slit image of the diodes is imaged on a photocathode of a streak tube in a...     

A fluorometer for measuring a particular fluorescence emanating from a specimen and operating in accordance with the following method. Producing a burst of concentrated light energy and directing the concentrated light energy toward the specimen to produce a fluorescence from the specimen including the...     

An assay technique for the qualitative and/or quantitative detection of a chemical, biochemical or biological species in a sample comprising (a) coating at least a predetermined part of a pre-formed surface with a thin film of a material capable of binding the species to be assayed, said thin film incorporating...     

A method of measuring thin film thickness, especially on semiconductor substrates, in which the substrate is illuminated with ultraviolet light of a fixed wavelength corresponding to a persistent spectral line and the amount of light reflected from the substrate is detected and measured. The ultraviolet...     

An assay technique for qualitative and quantitative detection of a chemcial, biochemical or biological detection of a chemical, biochemical or biological species in sample. The technique comprises (a) coating at least a predetermined part of a pre-formed surface on a substrate with a thin film of a material...     

A frequency domain spectrofluorometer employing extrinsically modulated DC light sources or intrinsically modulated laser pulse trains, containing multiple, harmonically related, phase-locked frequencies for exciting a fluorescent sample. High frequency light emissions of the excited sample are simultaneously...     

A method and means for detecting the presence of infrared radiation and/or he presence of an optical dielectric transmitting element, such as a lens or window, is provided. By correlating the radiation wavelength with a visible detector wavelength and a specific dielectric element, the element can be...     

A scanning spectrometer scans the spread spectrum of sunlight and low level luminescence or fluorescence past a slit to provide the spectrum scanned in the time domain. An image intensifier responsive to the light energy passing through the slit is gated on when Fraunhofer lines are coincident with the...     

A sensor uses the principle of surface plasmon resonance (SPR) to monitor the progress of the reaction between a sample and a sensitive layer (for example an antibody layer). The layer is applied to the rear surface of a metallic film formed on the surface of an optically transmissive component in the...     

A noninvasive pulsed fluorescence gas velocimeter that determines dynamic parameters of a gas flow from detection of fluorescent re-radiation of excited molecules of the gas. The present invention has higher altitude capability than devices that utilize particulate scattering. The velocimeter requires...     

A coupling interface between a two components of a spectrometer system having a monochromator, a spectrometer and a fiber cable therebetween includes a focusing lens and two crossed cylindrical lenses. The focusing lens is used to assure that the convergence angle of light entering the fiber is within...     

A time-resolved infrared spectrophotometer capable of investigating samples exhibiting short periods of response to stimuli. The spectrophotometer comprises a rapid scan interferometer, a stimulus generator periodically producing a stimulus not in synchronism with the reference signal produced by the...     

A surface plasmon resonance (SPR) sensor in which a membrane is used to enable the sensitive material to be readily changed to enable tests to be carried out on a sequential basis simply by moving or changing the membrane. In one embodiment, the membrane takes the form of a continuous film (24) which...     

A luminescence measuring system is provided for detecting luminescence at extremely low concentrations of luminescing moieties. The method employs alternating radiation at a plurality of loci of an inhomogenous solution, where the radiant power is maintained constant, and the irradiated volumes of pairs...     

A technique for examining surface structures which differ in respect of refractive index and/or height modulation of the surface comprises introducing these surface structures into a plasmon surface polariton field and scanning them by means of surface plasmon microscopy.

A laser scanning microscope produces molecular excitation in a target material by simultaneous absorption of two photons to thereby provide intrinsic three-dimensional resolution. Fluorophores having single photon absorption in the short (ultraviolet or visible) wavelength range are excited by a stream...     

A surface plasmon resonance (SPR) sensor is adapted for biochemical and similar testing on large area samples such as the gel of an electrophoresis apparatus. The gel is sandwiched between a pair of plates. One of the plates is of transparent material and, sandwiched between itself and the gel is a metal...     

In a laser-operated detecting device, the light source of a laser beam applied to a specimen under test is a tunable laser whose wavelength can be continuously tuned in a certain range. The detecting device is realized as a high speed liquid chromatography fluorescence detector, a high speed liquid chromatography...     

Disclosed is a process and apparatus to analyze metal particles to determine their composition and to generate a sorting signal. The particles are exposed to a pulsed laser beam by which they are partly vaporized to form a plasma so that the particles are cleaned and the cleaned area is subsequently...     

A biological sensor which utilizes the phenomenon of surface plasmon resonance to detect the refractive index change which occurs when two components--for example antibody and corresponding antigen--react with one another. Surface plasmon resonance takes place at the sloping exit surface of an optical...     

A concentration detector having a light source that is responsive to a chemical absorption peak to produce substantially monochromatic light at a wavelength of this absorption peak. The wavelength can be stabilized against an absorption peak in a reference chemical or by an absorption peak in a sample...     

The present invention is drawn to a surface plasmon resonance (SPR) sensor in which the phenomenon of long-range surface plasmon resonance is used to develop a highly sensitive detector for use in biological, biochemical or general chemical testing. The sensor includes a laminar structure having of a...     

A time-resolved fluorometer, having a light tight enclosure, for detecting the presence of an analyte in a sample. Within the light tight enclosure are a pulsed dye laser that produces a pulsed light beam for sample excitation, a light tight sample excitation station through which samples, treated with...     

An ambient surface contamination sensor including a source for supplying gas, which is energized before it reaches the surface being sensed so that it is capable of transferring energy to the contaminants on the surface or vapor originating therefrom. The sensor then detects the optical emission from...     

A surface plasmon resonance (SPR) detector includes a laser source for generating radiation which is reflected off a concave reflector to a point or line of incidence on the interface between a glass slide and a metal layer. A single pencil beam from the laser is scanned by a moving mirror across a small...     

A wavelength of light emitted from a semiconductor laser is shifted to a shorter wavelength with wavelength converting means and the resulting light of a shorter wavelength is applied to a sample. Upon exposure to the light of the shorter wavelength, the sample emits light of interest and its waveform...     

A converter for using a spectrophotometer as a fluorometer includes a barrier for blocking the light in a collimated beam from reaching the detector of the spectrophotometer after this light has passed through and excited a sample material. A second detector is positioned to receive any fluorscence from...     

An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum...     

An assay technique for qualitative and quantitative detection of a chemical, biochemical or biological detection of a chemical, biochemical or biological species in sample. The technique comprises: (a) coating at least a predetermined part of a pre-formed surface on a substrate with a thin film of a...     

The illuminating optical system comprises a light source for emitting a light beam having an elongated sectional shape in parallel with the optical axis thereof, and a condenser lens for focusing said light beam onto a sample. This illuminating optical system permits easily correcting aberrations in...     

A velocimeter measures flow characteristics of a flow travelling through a chamber in a given direction. Tracer particles are entrained in the flow and a source of radiant energy produces an output stream directed transverse the chamber and having a sufficient intensity to vaporize the particles as they...     

A photometric apparatus is provided for analyzing a sample liquid capable of fluorescing when exposed to excitation light. The sample liquid, surrounded by a sheath liquid under conditions of laminar flow to form an interface between the sample liquid and the sheath is passed through a cell conduit....     

The fluorescence spectrophotometer automatically sets the response time at the most suitable value for every sample, thus accurate quantitative measurements can be made with minimum measuring time. The response time characterizes the filtering operation by which noises are eliminated from the primary...     

An optical inspection system for using laser-induced luminescence to detect the quality of materials, such as fuel. The inspection system comprises an excitation means for illuminating a specimen to cause it to produce fluorescent radiation. The spectral representation of the fluorescence produced by...     

A coverslip holder assembly for a front face fluorimetry flow cell has a holder body and a hingedly attached clamp for releasably maintaining a coverslip adjacent the front face of the holder body and flow cell. The assembly is preferably a unitary arrangement wherein the clamp is hingedly mounted adjacent...     

Apparatus for emission spectrochemical analysis of a sample containing elements to be analyzed, wherein the sample is cyclically excited with relatively high energy to vaporize the sample elements and successively with relatively low energy to cause the vaporized elements to emit light containing spectral...     

A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin...     

A reflection fluorometer has a cylindrical measuring optical waveguide with a capillary-like structure which permits a beam of pulsed light in the form of a conical envelope to be directed onto a front face of the measuring optical waveguide. The reflection fluorometer comprises a device for timed measurement...     

A Fourier transform spectrometer for conducting Fourier transform spectroscopy. The spectrometer has a light source emitting light to a sample via a rapid scan interferometer. The transmittance of the sample varies when stimulated. A stimulus generator gives a stimulus to the sample at regular intervals...