Broadly this invention provides inbred corn line ZS01429. The methods for producing a corn plant by crossing the inbred line ZS01429 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01429 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01429 with at least one other corn line.
Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced.
The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance.
Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F.sub.2 population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection.
Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc.
To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F.sub.1 generation and self pollinating to produce a F.sub.2 generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids.
SUMMARY OF THE INVENTION
The present invention relates to an inbred corn line ZS01429. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01429.
Generally then, broadly the present invention includes an inbred corn seed designated ZS01429. This seed produces a corn plant.
The invention also includes the tissue culture of regenerable cells of ZS01429 wherein the tissue regenerates plants having the genotype of ZS01429. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01429 having ZS01429's genotype.
The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01429 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01429 and plants of another inbred line. Hybrid plants grown from this hybrid seed.
The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01429 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed.
A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01429 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above.
DEFINITIONS
In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided.
BL MOIST
The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity.
COLD GERM
Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating.
ECB
European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers.
EMERGE
The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves.
GI
This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula:
GI=100+0.5(YLD)-0.9(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR)
GLS
Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*
GW
Goss' Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*
HEATP10
The number of Growing Degree Units (GDU's) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU's for a 24 hour period are: ##EQU1##
The highest maximum temperature used is 86.degree. F. and the lowest minimum temperature used is 50.degree. F. For each inbred or hybrid it takes a certain number of GDU's to reach various stages of plant development.
HEATBL
The number of GDU's after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer).
HEATPEEK
The number of GDU's after planting of an inbred when approximately 50 percent of the plants show visible tassel extension.
HEATP50 or HTP50
The number of GDU's required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.
HEATP90
The number of GDU's accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.
HEATS10
The number of GDU's required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.
HEATS50 or HTS50
The number of GDU's required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.
HEATS90
The number of GDU's required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.
MDMV.sub.A
Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*
MDMV.sub.B
Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a "1" being very susceptible and a "9" being very resistant.*
MOISTURE
The average percentage grain moisture of an inbred or hybrid at harvest time.
NLB
Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*
PCT TILLER
The total number of tillers per plot divided by the total number of plants per plot.
PLANT
This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like.
PLANT HEIGHT
The distance in centimeters from ground level to the base of the tassel peduncle.
RM
Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System.
SHED
The volume of pollen shed by the male flower rated on a 1-9 scale where a "1" is a very light pollen shedder, a "4.5" is a moderate shedder, and a "9" is a very heavy shedder. The Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale. Any shed on Table 3 can be multiplied by 3 to reach the 1-9 shed scale.
SLB
Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*
TWT
The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture.
VIGOR
Visual rating of 1 to 9 made 2-3 weeks post-emergence where a "1" indicates very poor early plant development, and a "9" indicates superior plant development.
WARM GERM
A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating.
YIELD (YLD)
Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre.
% DROPPED EARS (DE)
The number of plants per plot which dropped their primary ear divided by the total number of plants per plot.
% LRG FLAT
Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings. % LRG ROUND
Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings.
% MED FLAT
Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings.
% MED ROUND
Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings.
% SML FLAT
Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings.
% SML ROUND
Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings.
% ROOT LODGE (RL)
Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot.
% STALK LODGE (SL)
Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot.
Resistant--on a scale of 1-9 with 9 evidencing the trait most strongly: 1-2.9 ratings are susceptible, 3-5.9 ratings are intermediate, and 6-9 ratings are resistant.
DETAILED DESCRIPTION OF THE INVENTION
ZS01429 can be used as a male line due to its pollen shed. This inbred can be a female but it makes large seed which is not preferred in the central and northern corn belts. This ZS01429 line evidences good general combining ability. This line produces corn plants with reddish silks that come out prior to tassel shed.
The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Slater, Iowa.
The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01429.
The best method of producing the invention, ZS01429 which is substantially homozygous, is by planting the seed of ZS01429 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen.
TABLE 1
__________________________________________________________________________
ZS01429
VARIETY DESCRIPTION INFORMATION
__________________________________________________________________________
#1 Type: Dent
#2 Region Best Adapted: northern and central regions
INBRED WB:ZS01429
#3 MATURITY
DAYS HEATUNITS
78 1392 FROM PLANTING TO 50% OF PLANTS IN SILK
78 1392 FROM PLANTING TO 50% OF PLANTS IN POLLEN
5 FROM 10% TO 90% POLLEN SHED
#4 PLANT
DATA
4 ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3
= MODERATE 4 = DARK
#5 LEAF
COLOR/DATA
2/MEDIUM GREEN LEAF COLOR **MUNSELL CODE-5GY 4/4
9 LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH
FUZZ)
5 MARGINAL WAVES (1 = NONE TO 9 = MANY)
5 LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)
#6 TASSEL
COLOR/DATA
7 POLLEN SHED (0 = STERILE TO 9 = HEAVY SHEDDER)
7/YELLOW ANTHER COLOR **MUNSELL CODE-5Y 8/8
1 & 12/ GLUME COLOR **MUNSELL CODE-2.5GY 7/6 w/s 5R 6/6
2 BAR GLUME: 1 = ABSENT 2 = PRESENT
#7A EAR (UNHUSKED DATA)
COLOR/DATA
17/PURPLE SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-5R
5/6
1/LIGHT GREEN FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL
CODE-SGY 7/8
22/TAN DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL
CODE-2.5Y 8/4
1 POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2 =
HORIZONTAL 3 = PENDENT
7 HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT)
3 HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8CM
3 = 8-10CM 4 = >10 CM
#7B EAR (HUSKED DATA)
DATA
2 KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT
2 ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 =
SPIRAL
2 EAR TAPPER: 1 = STRAIT 2 = AVERAGE 3 = EXTREME
#8 KERNEL (DRY)
COLOR/DATA
1 ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG
8/YELLOW-ORANGEALEURONE COLOR **MUNSELL CODE-2.SGY 7/10
8/YELLOW-ORANGEHARD ENDOSPERM COLOR **MUNSELL CODE-2.5Y 8/10
3 ENDOSPERM TYPE
7/YELLOW CROWN COLOR **MUNSELL CODE-2.5Y 8/10
#9 COB
COLOR
12/LIGHT RED COB COLOR **MUNSELL CODE-10R 7/6
__________________________________________________________________________
1995 PROJECT WB PVP TRAITS
INBRED ZS01429
#10 N MEAN STD. T-STAT PROB 95%
__________________________________________________________________________
CI
EAR HEIGHT(CM) 15 72.65 5.90 47.67 0.0000
(69.66, 75.63)
LENGTH OF PRIMARY EAR LEAF(CM)
15 90.10 5.68 61.48 0.0000
(87.23, 92.97)
WIDTH OF PRIMARY EAR LEAF(CM)
15 8.12 0.45 70.02 0.0000
(7.89, 8.35)
TOP EAR INTERNODE(CM) 15 10.51 1.02 39.86 0.0000
(9.99, 11.02)
DEGREE OF LEAF ANGLE 15 18.33 2.44 29.10 0.0000
(17.10, 19.57)
# OF EARS PER PLANT 15 1.07 0.26 16.00 0.0000
(0.94, 1.20)
# OF LEAVES ABOVE TOP EAR
15 5.00 0.53 36.23 0.0000
(4.73, 5.27)
# OF PRIMARY LATERAL TASSEL BRANCHES
15 8.13 1.60 19.72 0.0000
(7.32, 8.94)
PLANT HEIGHT(CM) 15 173.7 9.66 69.62 0.0000
(168.8, 178.5)
TASSEL LENGTH(CM) 15 32.70 3.18 39.78 0.0000
(31.09, 34.31)
TASSEL BRANCH ANGLE 15 37.33 4.17 34.68 0.0000
(35.22, 39.44)
# OF TILLER PER PLANTS 15 0.00 0.00 (0.00, 0.00)
WEIGHT PER 100 KERNELS(GM)
15 31.03 1.75 68.59 0.0000
(30.14, 31.91)
EAR LENGTH(CM) 15 14.03 0.93 58.14 0.0000
(13.56, 14.51)
EAR WEIGHT(GM) 15 89.29 10.84
31.89 0.0000
(83.80, 94.77)
# OF KERNEL ROWS 15 10.53 1.41 28.99 0.0000
(9.82, 11.25)
COB DIAMETER AT MID-POINT(MM)
15 27.87 1.73 62.51 0.0000
(26.99, 28.74)
EAR DIAMETER AT MID-POINT(MM)
15 43.53 1.77 95.39 0.0000
(42.64, 44.43)
KERNEL LENGTH(MM) 15 11.53 0.64 69.80 0.0000
(11.21, 11.86)
KERNEL THICKNESS(MM) 15 4.73 0.46 40.05 0.0000
(4.50, 4.96)
KERNEL WIDTH(MM) 15 10.13 0.52 76.00 0.0000
(9.87, 10.39)
% ROUND KERNELS(SHAPE GRADE)
15 52.27 10.43
19.41 0.0000
(46.99, 57.54)
SHANK LENGTH 15 6.77 1.53 17.09 0.0000
(5.99,
__________________________________________________________________________
7.54)
#11 DISEASE RESISTANCE - Northern leaf blight = 6
NLSr2* = 7.5
Gray leaf spot = 3.5
Eye = 4.5
GW = 7
MDMVB = 7.5
__________________________________________________________________________
#12 Other inbreds comparable to the present invention are ZS0193, PVP
9200037 and Z50986, PVP 9200041.
__________________________________________________________________________
* Northern Leaf Spot Race Two
The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding.
The purity and homozygosity of inbred ZS01429 is constantly being tracked using isozyme genotypes as shown in Table 2.
Isozyme Genotypes for ZS01429
Isozyme data were generated for inbred corn line ZS01429 according to procedures known and published in the art. The data in Table 2 gives the electrophoresis data on ZS01429.
TABLE 2
__________________________________________________________________________
ELECTROPHORESIS RESULTS FOR ZS01429
INBRED
ACP1
ACP4
ADH
MDH1
MDH2
PGD1
PGD2
PH1
PGM
IDH2
__________________________________________________________________________
ZS01429
33 00 22 22 11 11 22 22 22 22
__________________________________________________________________________
Inbred and Hybrid Performance of ZS01429
The traits and characteristics of inbred corn line ZS01429 are listed to compare with other inbreds and/or in hybrid combination. ZS01429 data shows the characteristics and traits of importance, giving a snapshot of ZS01429.
Table 3A shows a comparison between ZS01429 and ZS0193. ZSO 1429 has improved emergence and seed yield compared to ZS0193ZSO 1429 produces larger size seed than ZS0193.
TABLE 3A
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
__________________________________________________________________________
PCT PLANT
EAR EAR PCT
YEAR INBRED
VIGOR EMERGE
TILLER
HEIGHT
HEIGHT
SHED QUALITY
BARREN
__________________________________________________________________________
OVERALL
ZS01429 92.8
ZS0193 52.2
# EXPTS 1
DIFF 40.6
PROB
__________________________________________________________________________
YEAR INBRED
HEATP10
HEATP50
HEATP90
HEATS10
HEATS50
HEATS90
__________________________________________________________________________
OVERALL
ZS01429
ZS0193
# EXPTS
DIFF
PROB
__________________________________________________________________________
BL % ROOT
% STALK
% DROPPED
YEAR INBRED
HEATPEEK
HEATBL
MOIST
LODGE
LODGE
EARS MOISTURE
YIELD
__________________________________________________________________________
OVERALL
ZS01429 2.7 0.0 1.8 11.0 65.1
ZS0193 0.2 0.0 0.2 11.0 57.6
# EXPTS 1 1 1 1 1
DIFF 2.5 0.0 1.6 0.1 7.5
PROB
__________________________________________________________________________
WARM COLD % LRG
% LRG
% MED
% MED % SML % SML
YEAR INBRED
GERM GERM ROUND
FLAT ROUND
FLAT ROUND FLAT
__________________________________________________________________________
OVERALL
ZS01429 36.3 37.8 16.3 8.0 0.5 0.3
ZS0193 26.8 35.2 22.0 13.1 1.0 0.7
# EXPTS 1 1 1 1 1 1
DIFF 9.5 2.6 5.8 5.1 0.5 0.4
PROB
__________________________________________________________________________
Table 3B compares ZS01429 with ZS0986. ZS01429 shows significantly better seedling vigor and emergence. ZS01429 silks significantly earlier at the S10, S50 and S90 stages, and sheds pollen significantly earlier then ZS0986 at the P50 and P90 stages. The PEEK of ZS01429 is significantly earlier than ZS0986, although pollen SHED is less. ZS01429 is significantly taller then ZS0986 but has equal ear height. There is a significantly greater percent of larger seed size(s) compared to ZS0986.
TABLE 3B
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
__________________________________________________________________________
PCT PLANT
EAR EAR PCT
YEAR INBRED
VIGOR EMERGE
TILLER
HEIGHT
HEIGHT
SHED QUALITY
BARREN
__________________________________________________________________________
OVERALL
ZS01429
6.2 88.1 146.3
69.6 4.8
ZS0193
4.8 74.6 130.6
69.6 6.4
# EXPTS
5 6 5 5 5
DIFF 1.4 13.5 15.7 0.0 1.6
PROB 0.025**
0.049** 0.002*
1.000
0.078***
__________________________________________________________________________
YEAR INBRED
HEATP10
HEATP50
HEATP90
HEATS10
HEATS50
HEATS90
__________________________________________________________________________
OVERALL
ZS01429
1430 1501 1596 1477 1509 1558
Z50193
1513 1554 1639 1516 1560 1609
# EXPTS
5 5 5 5 5 5
DIFF 83 53 44 40 51 51
PROB 0.153 0.025**
0.029**
0.053***
0.016**
0.048**
__________________________________________________________________________
BL % ROOT
% STALK
% DROPPED
YEAR INBRED
HEATPEEK
HEATBL
MOIST
LODGE
LODGE
EARS MOISTURE
YIELD
__________________________________________________________________________
OVERALL
ZS01429 2.7 0.0 1.8 11.0 65.1
OVERALL
ZS01429
1402 2456 33.0 2.7 0.0 1.8 12.0 46.1
ZS0986
1469 2523 30.0 0.0 0.0 2.4 11.4 43.4
# EXPTS
5 1 1 1 1 1 6 6
DIFF 67 67 3.0 2.7 0.0 0.6 0.6 2.7
PROB 0.070*** 0.167
WARM COLD % LRG
% LRG
% MED
% MED % SML % SML
YEAR INBRED
GERM GERM ROUND
FLAT ROUND
FLAT ROUND FLAT
__________________________________________________________________________
OVERALL
ZS01429
94.7 89.7 29.1 38.7 18.0 10.9 1.1 0.9
ZS0986
97.3 95.3 1.5 8.0 23.0 46.7 6.8 8.6
# EXPTS
5 5 6 6 6 6 6 6
DIFF 2.6 5.6 27.6 30.6 5.0 35.8 5.7 7.7
PROB 0.088***
0.074***
0.014**
0.000*
0.501
__________________________________________________________________________
Table 4 shows the GCA (general combining ability) estimates of ZS01429 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of "checks". The check data is from other companies' hybrids, particularly the leader in the industry and ICI Seeds' commercial products and pre-commercial hybrids which were grown in the same sets and locations.
Table 4A shows an average performance summary (GCA) of ZS01429 crossed to 1006 different inbreds. ZS01429 shows a positive advantage for yield of 0.5 and for moisture of 0.2. ZS01429 has a tendency to stalk lodge as evidenced by the -0.8 rating.
TABLE 4A
__________________________________________________________________________
GCA & SCA ESTIMATES
ENT
ZS01429
ADVANTAGES OVER COMMERCIALS & N'S
N93 N94
N95
N FI Y M
GI I YLD
MST
% SL
% RL
% DE
TWT
POP RM
__________________________________________________________________________
XR =
444
165
388
1006
0.1
0.1
-0.3
-0.0
0.5
0.2
-0.8
-0.1
0.1 -0.0
-155
108
XH = 79 -0.4
0.0
-1.3
0.2
-1.2
0.4
-0.8
-0.1
0.0 0.1
-177
109
XT = 18 1.9
0.3
0.8
4.8
3.6
0.4
-1.1
-0.0
-0.0
-0.6
-54 108
__________________________________________________________________________
XR = GCA ESTIMATE: WEIGHTED BY EXPT
XH = GCA ESTIMATE: WEIGHTED BY PARENT2
XT = SAME AS XH BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA
Table 4B compares ZS01429 in random hybrid combinations with ZS0193 and ZS0986ZS01429 has an improved yield moisture (YM) rating compared to both comparable inbreds. ZS0193 has a better yield advantage but a larger moisture disadvantage and ZS0986 has a very large yield disadvantage and a moisture advantage, thus, both comparable inbreds have a disadvantage in the YM rating. Stalk lodging and root lodging ratings of ZS01429 are less than ZS0193 but greater than ZS0986The GI index shows ZS01429 to be intermediate between ZS0193 and ZS0986.
TABLE 4B
__________________________________________________________________________
N FI YM GI I YLD MST
__________________________________________________________________________
ZS01429
XR =
637 -0.5
-0.0 0.1 0.1 0.5 -0.2
ZS0193
XR =
85 -1.0
-0.2 1.2 0.5 1.9 -1.0
ZS0986
XR =
1152 -3.1
-0.3 -3.7
-2.5 -4.4
0.2
__________________________________________________________________________
% SL % RL
% DE TWT POP RM
__________________________________________________________________________
ZS01429
XR =
-0.1 -0.1
0.0 -0.2
-178 109
ZS0193
XR =
0.2 0.1 0.0 -2.4
-519 101
ZS0986
XR =
-0.7 -0.9
0.0 0.8 -91 107
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Table 5 shows the yield response of ZS01429 in hybrid combination in comparison with the plants in the environment around it at the same location. ZS01429 in hybrid combination yields well across all environments, in particular at levels less than 150 bushels per acre. While stable at yield levels above 150 bushels, the ZS01429 hybrid has not demonstrated aggressive yield potential.
TABLE 5
______________________________________
YIELD RESPONSE
HYBRID YIELD
______________________________________
ZS01429/Inbred
79 104 128 152 176 200
Environment
75 100 125 150 175 200
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Table 6A shows the positive advantage of the ZS01429 hybrid to three commercially available ICI Seeds' hybrids and one major competitive hybrid. The advantage is particularly striking for GI index and yield compared to all four hybrids. The ZS01429 hybrid has an equal or slight advantage over all four hybrids in percent stalk and root lodging and dropped ears. ZS01429 has lower grain test weight (TWT) compared to all four hybrids.
TABLE 6A
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HYBRID SUMMARY
ZS01429/INBRED
PERFORMANCE DATA
HYBRID N FI GI YLD
MST % SL
% RL
% DE
TWT
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RE 8704
71
4.7 1.8
3.4
1.3 0.0
0.1 0.0 -0.7
RE 8700
71
-0.5
4.5
2.7
-2.2 1.1
0.1 0.2 -0.2
RE 8692lt
34
6.6 4.2
7.1
1.0 0.2
0.4 0.0 -2.0
RE Competitor
50
1.2 1.6
1.3
-0.2 0.1
0.6 0.0 -2.2
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*significant difference at the 0.1 level
Table 7A shows the entomology strengths and weaknesses of the present inbred.
TABLE 7A
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EAR PLANT
ECB1 ECB2 DAMAGE INTEGRITY
INBRED RATING RATING RATING RATING
______________________________________
ZS01429 5.2 5.4 2.7 5.8
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In hybrid production, the inbred ZS01429 should be used primarily as a male due to the good pollen shed and large size of the kernels. ZS01429 has very good late season plant health, very erect leaves and long, narrow ears with 1012 kernel rows. The inbred ZS01429 has red silks that emerge before pollen shed.
In hybrid combination, ZS01429 has good yield for moisture characteristics, which enables the hybrid to move into the southern and central regions of South Dakota, Minnesota, Wisconsin and Michigan. It is a versatile line and makes hybrids with good yield/moisture characteristics. Plant health of the ZS01429 hybrid is good in its region of adaptation. ZS01429 has good general combining ability, especially with inbreds of Lancaster and lodent backgrounds.
The foregoing is set forth by way of example and is not intended to limit the scope of the invention.
This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01429. Further, both first and second parent corn plants can come from the inbred corn line ZS01429. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01429 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like.
Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01429 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line ZS01429.
Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980's was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues.
European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982.
A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. All restrictions on availability to the public of such material will be removed upon allowance of any claims of this application by depositing at least 2500 seeds of this invention at the American Type Culture Collection, Rockville, Md. The Applicant made a deposit on Jun. 11, 1997, of at least 2500 seeds of Inbred Corn Line ZS01429 with the American Type Culture Collection (ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCC accession number is 209111. Additionally the Applicant has satisfied all of the requirements of C.F.R..sctn.1.801-1.809, including providing an indication of the viability of the sample. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period.
Inbreds designated LH are available from Holden's Foundation Seed, Inc. in Iowa. Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office.
Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein.
